
The Argentinean international was troubled by a tight hamstring over the weekend and, as a result, has not been named in the Reds' 18-man squad that left for Tuscany.
Mascherano is still expected to be available for selection for Sunday's big Premier League clash against Chelsea at Stamford Bridge.
But, with this game in mind, the club said the player 'will not be risked' against Fiorentina.
Liverpool go into the game against the Serie A side at the Stadio Artemio Franchi with a 1-0 victory already under their belts in European competition this season.
Rafael Benitez's team opened their Champions League Group E account with a relatively comfortable three points at home to Hungarian side Debrecen a fortnight ago.

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