The number eight picked up the injury earlier in the week and his withdrawal from the team sees Andy Powell switch to the back of the scrum, with flanker Dan Lydiate promoted into the starting XV.
In the absence of Jones, Gethin Jenkins will captain the team.
Commenting on the Jones injury, Wales physio Mark Davies said: 'Ryan pulled up in Tuesday's conditioning session with a back problem.
'This obviously needed time to settle and unfortunately the problem has not resolved in time for him to be available on Saturday.
'He has been receiving treatment and was unable to train yesterday, but today we have taken the decision that he is simply not going to be ready to take the field.'
Lydiate's promotion to the starting team means Sam Warburton will take his place on the bench having faced the Wallabies earlier this week when the tourists ran out 33-1 victors over Cardiff Blues.
In the absence of Jones, Gethin Jenkins will captain the team.
Commenting on the Jones injury, Wales physio Mark Davies said: 'Ryan pulled up in Tuesday's conditioning session with a back problem.
'This obviously needed time to settle and unfortunately the problem has not resolved in time for him to be available on Saturday.
'He has been receiving treatment and was unable to train yesterday, but today we have taken the decision that he is simply not going to be ready to take the field.'
Lydiate's promotion to the starting team means Sam Warburton will take his place on the bench having faced the Wallabies earlier this week when the tourists ran out 33-1 victors over Cardiff Blues.
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