Nick Evans helped himself to 19 points as Harlequins moved up to ninth place in the Guinness Premiership.
The hosts, who lost 26-17 at Franklins Gardens last week, welcomed back Danny Care and Ugo Monye from international duyt whilst Nick Easter returned to the side after a calf injury.
Gloucester made two changes from the side that beat Leicester 12-9 last Friday with Olly Morgan in at full-back, whilst Tom Voyce replaced Lesley Vainikolo on the wing.
Gareth Delve and Charlie Sharples scored unconverted tries either side of a converted George Lowe try for the home side.
Nick Evans and Freddie Burns grabbed two penalties a piece before Evans himself touched down for an unconverted try to give Quins an 18-16 half-time lead.
Easter and Lowe scored converted tries within ten minutes of the restart before Sharples' second try and a Nicky Robinson penalty brought Gloucester back into it.
Evans and Gloucester traded penalties in the last ten minutes but the hosts held on to go ahead of their opponents in the league table.
Elsewhere, Toby Flood inspired Leicester Tigers to a 39-6 win against bottom placed Leeds Carnegie at Welford Road.
Flood was in the Tigers' first XV for the first time since he ruptured his Achilles in last season's Heineken Cup semi-final versus Cardiff Blues.
Lewis Moody and Tom Smith returned from international duty whilst Matt Smith replaced Johne Murphy on the wing.
Rock-bottom Leeds, who threw away a huge lead against Sale last week, made one change with Rhys Oakley in at number eight in place of Alfie To'oala.
Flood and Joe Ford each kicked two penalties as the first half finished 6-6 with each side losing a player for ten minutes as Moody and Calum Clark were sin-binned.
The floodgates opened in the second half as Flood, Brett Deacon, Anthony Allen, Jeremy Staunton and a penalty try plus three conversions from Flood and one from Staunton gave the Tigers an emphatic win.
The win puts Leicester fourth whilst Leeds remain rooted to the foot of the table.
The hosts, who lost 26-17 at Franklins Gardens last week, welcomed back Danny Care and Ugo Monye from international duyt whilst Nick Easter returned to the side after a calf injury.
Gloucester made two changes from the side that beat Leicester 12-9 last Friday with Olly Morgan in at full-back, whilst Tom Voyce replaced Lesley Vainikolo on the wing.
Gareth Delve and Charlie Sharples scored unconverted tries either side of a converted George Lowe try for the home side.
Nick Evans and Freddie Burns grabbed two penalties a piece before Evans himself touched down for an unconverted try to give Quins an 18-16 half-time lead.
Easter and Lowe scored converted tries within ten minutes of the restart before Sharples' second try and a Nicky Robinson penalty brought Gloucester back into it.
Evans and Gloucester traded penalties in the last ten minutes but the hosts held on to go ahead of their opponents in the league table.
Elsewhere, Toby Flood inspired Leicester Tigers to a 39-6 win against bottom placed Leeds Carnegie at Welford Road.
Flood was in the Tigers' first XV for the first time since he ruptured his Achilles in last season's Heineken Cup semi-final versus Cardiff Blues.
Lewis Moody and Tom Smith returned from international duty whilst Matt Smith replaced Johne Murphy on the wing.
Rock-bottom Leeds, who threw away a huge lead against Sale last week, made one change with Rhys Oakley in at number eight in place of Alfie To'oala.
Flood and Joe Ford each kicked two penalties as the first half finished 6-6 with each side losing a player for ten minutes as Moody and Calum Clark were sin-binned.
The floodgates opened in the second half as Flood, Brett Deacon, Anthony Allen, Jeremy Staunton and a penalty try plus three conversions from Flood and one from Staunton gave the Tigers an emphatic win.
The win puts Leicester fourth whilst Leeds remain rooted to the foot of the table.
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